Table 1.

Taxonomic affiliations of top-scoring BLAST hits of Thermotogales ORFs found in the GenBank nonredundant (nr) database

	Tt. maritima	Tt. petrophila	Tt. lettingae	Ts. melanesiensis	F. nodosum
Bacteria	1,379 (74%)	1,355 (76%)	1,633 (80%)	1,345 (71%)	1,369 (78%)
Firmicutes	821 (44%) [66]	816 (46%) [65]	985 (48%) [59]	794 (42%) [60]	844 (48%) [58]
Class Clostridia	680	670	785	644	710
Order Thermoanaerobacterales	273	269	279	217	259
Class Bacilli (Order Bacillales only)	117	119	174	126	111
Proteobacteria	211	207	276	247	215
Aquificae	46	43	36	38	45
Chloroflexi	61	52	52	23	31
Deinococcus-Thermus	37	35	29	23	32
Bacteroidetes	30	32	40	38	29
Cyanobacteria	43	40	44	42	36
Actinobacteria	26	25	46	22	18
Planctomycetes	22	16	28	21	15
Acidobacteria	10	7	16	10	9
Spirochaetes	10	12	14	12	15
Archaea	204 (11%)	197 (11%)	187 (9%)	168 (9%)	135 (8%)
Euryarchaeota	171	155	148	138	111
Thermococcales	95	80	66	51	58
Archaeoglobales	18	18	13	13	7
Methanococcales	18	17	13	20	12
Methanosarcinales	20	21	30	29	16
Crenarchaeota	27	35	31	26	22
Thermoproteales	12	15	13	9	12
Desulfurococcales	8	12	10	11	3
Sulfolobales	5	6	6	4	6
Unclassified Archaea	6	7	8	4	2
Eukaryotes	16	17	13	12	11
Viruses	1	1	0	5	2
Others	6	5	6	6	1
Thermotogales specific*	252	210	201	343	232
ORFans†	52	22	81	170	71

All percentage values are fractions of the total number of ORFs for that genome. Numbers refer to the number of ORFs in each taxonomic category (only selected major contributors are shown). Within each major taxonomic group only the groups with largest number of genes are shown. Numbers in brackets indicate the percentage of thermophilic Firmicutes among the reported number of top-scoring BLAST hits to Firmicutes.

*, Homologs found in Thermotogales genomes, but not in the nr database.

†, Homologs found neither in the other Thermotogales genomes nor in the nr database.