Fig. 1.
eNOS is activated by SDF-1α. (A) Western blot analysis of eNOS activity in BAECs treated with 50 ng/mL SDF-1α for indicated time periods. (B) Western blot analysis of BAECs treated with SDF-1α with the indicated dosages for 5 min. (C) Results of a scratch wound-healing assay performed on BAECs pretreated with NG-monomethyl-l-arginine (l-NMMA) with or without the addition of 50 ng/mL SDF-1α. *, P < 0.05, compared with control cells without SDF-1α. #, P < 0.05, compared with control cells with SDF-1α. (D) Results of the Boyden chamber assay with BAECs pretreated with l-NMMA and stimulated with 50 ng/mL SDF-1α. *, P < 0.05, compared with control cells without SDF-1α. #, P < 0.05 compared with control cells with SDF-1α. (E) Western blot analysis of eNOS protein level in BAECs transfected with eNOS siRNA1, -2, -3, or a mixture of the three. (F) Results of a Boyden chamber assay using BAECs transfected with eNOS siRNA or control siRNA and stimulated with 50 ng/mL SDF-1α. *, P < 0.05, compared with control cells without SDF-1α. #, P < 0.05 compared with control cells with SDF-1α.