Table 2.

Iron parameters following acute phenylhydrazine-treated mice

	Nramp1+/+	Nramp1−/−
Transferrin saturation, normal iron diet, %	59 ± 5.2	38* ± 3.6
Transferrin saturation, low iron diet, %	56.2 ± 4.6	31.4* ± 2.3
Spleen index	0.015 ± 0.0027	0.021* ± 0.0035
Splenic iron content, μg/g wet weight	1236.4 ± 46.2	1487.4* ± 65.1
Liver iron content, μg/g wet weight	290.3 ± 14.2	310.4 ± 32.5
Hematocrit, %	27.4 ± 4.9	22.2* ± 3.1
Plasma erythropoietin, mU/ml	43.3 ± 6.7	207.9** ± 41.4

Acute hemolytic anemia was induced in 5-week-old Nramp1^+/+ and Nramp1^−/− mice with 2 consecutive daily 50 mg/kg injections of phenylhydrazine (Phz). The mice were killed 48 h after the last injection, and their iron parameters were evaluated. Unless otherwise specified, animals were maintained on an iron-replete diet (*, P ≤ 0.05; **, P ≤ 0.01; n = 6–8).