Table 3.

Iron parameters of chronic phenylhydrazine-treated mice

	Nramp1+/+	Nramp1−/−
Hepcidin mRNA, arbitrary units normalized to control	0.04 ± 0.001	0.008* ± 0.0002
Transferrin saturation, %	37 ± 5.3	28* ± 5.8
Spleen index	0.017 ± 0.0021	0.021* ± 0.0009
Splenic iron content, μg/g wet weight	1167.9 ± 42.2	1408.1* ± 31.9
Liver iron content, μg/g wet weight	228.3 ± 18.4	260.4 ± 26.9
Hematocrit, %	35.4 ± 1.7	30.7* ± 2.1
Plasma erythropoietin, mU/ml	103.8 ± 12.5	203.48** ± 9.7

Chronic hemolytic anemia was induced in 5-week-old Nramp1^+/+ and Nramp1^−/− mice with biweekly injections of 25 mg/kg of phenylhydrazine (Phz) for 4 weeks. The mice were killed, and their iron parameters were evaluated (*, P ≤ 0.05; **, P ≤ 0.01; n = 6–8).