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. 2009 Mar 17;106(14):5978–5983. doi: 10.1073/pnas.0811231106

Fig. 4.

Fig. 4.

Heat shock-dependent switching of AtTDX oligomerization and in vitro activity. (A) Changes in AtTDX oligomeric status in response to various heat shock treatments for 30 min, as analyzed by SEC. (Inset) The oligomeric status of AtTDX was confirmed by analysis on a 10% native PAGE gel (Upper) or a 12% SDS/PAGE gel (Lower), followed by silver staining. (B) Holdase chaperone, foldase chaperone, and DTNB reductase activities of heat-treated AtTDX were compared with those of native AtTDX incubated at 25 °C, which were set to 100%. (C and D) Heat shock-dependent changes of AtTDX hydrophobicity were measured by bis-ANS binding (C) and tryptophan fluorescence (D). AtTDX was incubated at 25 °C (line 1), 45 °C (line 2), 50 °C (line 3), 55 °C (line 4), and 60 °C (line 5) for 30 min. The fluorescence spectrum of 10 μM bis-ANS incubated alone at 60 °C is shown by line 6.