Skip to main content
. Author manuscript; available in PMC: 2009 Apr 8.
Published in final edited form as: Epigenetics. 2009 Jan 28;4(1):47–53. doi: 10.4161/epi.4.1.7288

Figure 1.

Figure 1

Representative immunoblots showing detection of H3K9/14Ac, H3K9Me3 and H4 in the nuclear (N) but not in the cytosolic (C) extract of the mouse whole brain. Nuclear (5 μg) and cytosol lysates (25 μg) were separated on polyacrylamide-SDS gels, transferred to nitrocellulose membranes, and immunoblotted for H3K9/14Ac and H3K9Me3 as well as total H4 as described in the Materials and Methods section. In the left panel, nuclear and cytosolic proteins and recombinant histone H3 and H4 were stained with Coomassie Blue (CB). In the western blot on the right the H4 was from the same recombinant source.