Table 3.
H3 acetylation and trimethylation in the combined preoptic area and hypothalamus during early development
| H3K9/14Ac | H3K9Me3 | |||||||
|---|---|---|---|---|---|---|---|---|
| Sex | N | acH3 | H4 | % | 3mH3 | H4 | % | |
| E18 | M | 9 | 695.90 ± 121.03 | 38.83 ± 5.12 | 82.15 ± 8.66 | 684.00 ± 119.31 | 43.95 ± 8.64 | 104.70 ± 9.32 |
| F | 10 | 773.74 ± 87.66 | 35.89 ± 4.33 | 100.00 ± 4.22 | 596.03 ± 89.66 | 38.23 ± 6.71 | 100.00 ± 6.23 | |
| PN0 | M | 8 | 600.31 ± 77.38 | 31.05 ± 5.80 | 127.67 ± 19.26 | 469.97 ± 51.83 | 32.25 ± 4.66 | 105.98 ± 9.00 |
| F | 6 | 619.66 ± 34.82 | 40.24 ± 6.43 | 100.00 ± 2.82 | 547.18 ± 23.00 | 41.11 ± 3.65 | 100.00 ± 2.72 | |
| PN6 | M | 10 | 539.59 ± 51.80 | 19.96 ± 1.82 | 107.60 ± 6.88 | 626.78 ± 70.18 | 19.63 ± 3.71 | 110.97 ± 9.05 |
| F | 7 | 660.02 ± 110.49 | 27.44 ± 4.19 | 100.00 ± 9.42 | 738.99 ± 76.98 | 28.72 ± 5.89 | 100.00 ± 8.09 | |
Intensities of acetylated and trimethylated H3 and total H4 measured by densitometry of histone western blots were expressed as mean ± SEM. Levels of H3K9/14Ac and H3K9Me3 were expressed as ratios normalized to total H4 and then set as the percentage relative to the females of the same ages (as 100%). E, embryonic day; PN, postnatal day; PN0, the day of birth; M, males; F, females.