Figure 1.

Trichostatin activation and reactivation of viral transgene expression. Silenced, rAAV/CMVlacZ stably transduced HeLa cells (CTL) were treated with 3 μM TSA for 24 hours (d1). After treatment, cells were washed thoroughly and were cultured for 2 weeks. Cells were stained with 5-bromo-4-chloro-3-indolyl β-d-galactoside at indicated days after TSA removal (d2 to d15). Expression of LacZ gradually decreased and completely disappeared after 2 weeks. LacZ expression was dramatically reactivated in cells that were treated again for 24 hours with 3 μM TSA (2-d1).