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Acetylation of specific histone H4 lysine residues at the transduced gene locus by chromatin immunoprecipitation (CHIP)–PCR assay. Chromatin immunoprecipitation and PCR analysis of the lacZ transgene, endogenous APRT, and β-globin genes after TSA treatment and removal. Polyclonal antibodies to tetraacetylated histone H4 or to individual, acetylated histone H4 lysines were used.
