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. Author manuscript; available in PMC: 2009 Apr 8.
Published in final edited form as: J Immunol. 2007 Sep 1;179(5):2999–3011. doi: 10.4049/jimmunol.179.5.2999

FIGURE 3.

FIGURE 3

Effect of NF-κB inhibitors on the expression of FcRn. Results are representative of three independent experiments. Ratios of FcRn to GAPDH mRNA are indicated below. A and B, Effects of CAPE on expression of FcRn mRNA. THP-1 cells were incubated with or without the NF-κB -specific inhibitor CAPE (25 μg/ml) for 2 h. THP-1 cells were subsequently stimulated with or without TNF-α. At the end of the incubation periods for the indicated times, RNA was isolated and analyzed by semiquantitative RT-PCR (A) or quantitative real-time RT-PCR (B). C, Effect of NF-κB blockage by IκBα on expression of FcRn. Human THP-1 cells were transfected with the dominant negative construct pCMV-IκBα (S32A/S36A). RNA was isolated and analyzed by semiquantitative RT-PCR.