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. Author manuscript; available in PMC: 2009 Apr 8.
Published in final edited form as: J Immunol. 2007 Sep 1;179(5):2999–3011. doi: 10.4049/jimmunol.179.5.2999

FIGURE 5.

FIGURE 5

Analysis of NF-κB binding. DNA binding was performed with nuclear extracts from THP-1 cells treated with or without TNF-α (50 ng/ml) for the indicated times (20 or 60 min). A 24-bp fragment, spanning the NF-κB binding sequences corresponding to the FcRn intron 2 (+1104) (A), intron 4 (+5561) (B), or intron 4 (+9651) (C), was used as a biotin-labeled probe. A 100-fold molar excess of unlabeled oligonucleotide was added (lanes 4 and 8) for competition. Supershift experiments (lanes 5 and 6) were performed in the presence of the indicated Abs. Free-labeled probes are also indicated by an arrow. n.s., Nonspecific bands.