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. Author manuscript; available in PMC: 2009 Apr 8.
Published in final edited form as: J Immunol. 2007 Sep 1;179(5):2999–3011. doi: 10.4049/jimmunol.179.5.2999

FIGURE 7.

FIGURE 7

NF-κB binding sequences from FcRn introns can enhance the transcription of the luciferase gene. A, Schematic representation of constructs pLuc-MCS containing NF-κB binding sequences from human FcRn introns 2 and 4. The pLuc-MCS plasmid has the minimal promoter with a TATA box. The plasmids pLuc-MCS +1104, pLuc-MCS +5561, pLuc-MCS +9651, and pLuc-MCS +3463, were constructed as described in Materials and Methods. The NF-κB sequences from FcRn introns are underlined. Numbers represent the locations of NF-κB sites in the human FcRn gene. A DNA sequence corresponding to +3463 in Fig. 4B was used as a negative control. B, HT-29 monolayers were cotransfected with the pLuc-MCS reporter plasmids as indicated and the NF-κB transactivator plasmid p50/65 in addition to the Renilla luciferase pRL-TK control plasmid. The pLuc-MCS backbone serves as a negative control. Luciferase activity was measured 24 h posttransfection. The results represent the mean of three independent experiments.