Fig. 2.

Inhibition of JAK3 and mTOR signaling blocks the effect of IL-2 on ionomycin-induced hyporesponsiveness in T cells. A. Th1 cells were treated with 1μM ionomycin for 16 hours in the presence of the 100μM of the JAK3 inhibitor WHI-P131 (JKI), 100nM of the PI3K inhibitor wortmannin (Wort), 1μM of the mTOR inhibitor rapamycin (Rap) or 20 μM of the p38 inhibitor SB203580 (p38I). Cells were then washed, rested for 6 hours and re-stimulated with immobilized anti-CD3 and anti-CD28 for 24 hours. Supernatants were collected and IL-2 expression determined by ELISA. B. Th1 cells were cultured with the same inhibitors in the absence of ionomycin and processed as described in A. Values are mean+SEM of 5 independent experiments. *p<0.05