Table 3.
Comparison of apoptotic assays from sputum neutrophils
| Method 1 | Method 2 | Method 3 | |||
| % Apoptotic neutrophils1 | % Av+/PI- | % Av+/PI+ | % Av-/PI+ |
% Tunel +ve cells2 |
|
| ALL SUBJECTS | 10.9 (1.22) |
17.19 (2.85) |
37.63 (5.27) |
21.67 (4.02) |
28.56 (4.09) |
Method 1: Microscopic morphological identification. Method 2: Annexin V/Propidium iodide (PI) staining. Method 3: Identification of DNA fragmentation (Tunel assay).
% AV+/PI-: early apoptosis, % AV+/PI+: later stage apoptosis/secondary necrosis and % AV-/PI+: necrosis.
1Significantly different compared to Tunel +ve cells (p < 0.001), % AV+/PI+ (p < 0.001) and % AV-/PI+ neutrophils using Tukey's multiple comparison post-hoc test. 2Significantly different compared to % AV+/PI- cells (p < 0.05).
Data are expressed as mean (SEM)