Table 3.
Coefficient of Variationa (CV) and Ct difference (ΔCt = Ctmax-Ctmin) determined on raw Ct values obtained by 18 primer pairs which amplified specific or multiple (m) gene transcripts of four gene families (Actin, α-tubulin, Translation elongation factor and ADP-ribosylation factor) in five data-sets.
| Unigene cluster | Gene annotation | I Data setb | II Data setc | III Data setd | IV Data sete | V Data setf | |||||
| CV | ΔCt | CV | ΔCt | CV | ΔCt | CV | ΔCt | CV | ΔCt | ||
| Ta54225 | Actin | 3.87 | 3.01 | 4.14 | 2.90 | 1.83 | 1.21 | 1.99 | 1.14 | 2.95 | 1.57 |
| Actin (m) | Actin | 4.41 | 3.78 | 4.46 | 3.78 | 3.87 | 1.90 | 1.27 | 0.65 | 3.12 | 1.76 |
| Ta1868 | Actin | 5.37 | 4.81 | 5.87 | 4.81 | 2.10 | 1.26 | 3.28 | 2.36 | 2.67 | 1.96 |
| Ta4344 | Actin | 6.09 | 6.15 | 6.90 | 6.15 | 1.73 | 1.12 | 4.35 | 2.58 | 6.71 | 4.97 |
| Ta20863 | Actin | 6.65 | 6.50 | 7.26 | 6.65 | 3.03 | 2.40 | 3.81 | 2.43 | 7.56 | 6.42 |
| Ta53908 | Actin | 7.20 | 6.90 | 7.92 | 6.90 | 2.67 | 1.94 | 3.70 | 2.23 | 8.20 | 6.21 |
| Ta25534 | α-tubulin 5 | 4.65 | 4.14 | 4.79 | 4.14 | 3.16 | 2.25 | 2.75 | 1.90 | 2.98 | 2.31 |
| Ta54519 | α-tubulin 4 | 6.94 | 7.33 | 7.83 | 7.33 | 4.03 | 2.82 | 5.65 | 3.70 | 7.04 | 5.38 |
| α-tubulin (m) | α-tubulin | 7.01 | 6.42 | 7.82 | 6.42 | 3.31 | 1.63 | 3.84 | 1.61 | 8.25 | 5.41 |
| Ta53981 | α-tubulin 2 | 7.54 | 6.39 | 8.31 | 6.39 | 4.00 | 2.04 | 3.52 | 1.93 | 9.21 | 6.25 |
| Ta33558 | α-tubulin 3 | 7.77 | 7.02 | 8.59 | 7.02 | 4.09 | 2.56 | 5.21 | 3.52 | 9.37 | 7.02 |
| Ta5566 | α-tubulin 1 | 8.05 | 8.49 | 8.82 | 8.49 | 4.84 | 2.73 | 5.42 | 3.58 | 8.04 | 6.60 |
| Ta53964 | Translation EF 1α-subunit | 3.55 | 3.46 | 3.74 | 3.46 | 1.04 | 0.66 | 1.74 | 0.91 | 2.71 | 2.00 |
| TEF-1α (m) | Translation EF 1α-subunit | 4.53 | 4.09 | 5.08 | 4.09 | 2.20 | 1.17 | 2.77 | 1.49 | 4.01 | 2.60 |
| Ta659 | Translation EF 1α-subunit | 4.82 | 4.35 | 5.27 | 4.35 | 2.12 | 1.16 | 2.50 | 1.22 | 3.82 | 2.48 |
| Ta2291 | ADP-ribosylation factor | 4.54 | 3.46 | 4.85 | 3.46 | 2.02 | 1.27 | 1.38 | 0.70 | 3.86 | 2.55 |
| Ta45379 | ADP-ribosylation factor | 4.59 | 4.03 | 4.85 | 4.03 | 2.21 | 1.35 | 2.15 | 1.18 | 5.16 | 3.77 |
| ADP-RF (m) | ADP-ribosylation factor | 6.70 | 4.68 | 6.24 | 4.68 | 3.73 | 2.23 | 2.22 | 1.19 | 6.26 | 4.34 |
aCV = Standard deviation divided by mean and multiplied by 100 of MST (Mean Square Total) among tissues, biological experiments and technical replicates within each of the two experiments.
bAll the 24 samples including tissues, developmental stages and temperature treatments.
c18 tissues and developmental stages.
dSix samples consisting of two temperature treatments (4°C and 33°C) for 24 and 48 h and their controls.
eSix floral organs from fully emerged spikes.
fSix vegetative tissues and developmental stages (shoots, stems and leaves).