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Time from challenge to 50% mortality. 3-week-old Golden Syrian hamsters in groups of four were challenged by intraperitoneal inoculation with serial 10-fold dilutions of Leptospira kirschneri serovar Grippotyphosa, strain RM52. Each dose was administered to hamsters in groups of four. Each point represents the time to death of the second of four hamsters. The LD50 of this strain is <10 organisms.
