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. Author manuscript; available in PMC: 2009 Apr 8.
Published in final edited form as: Microbiology (Reading). 2006 Dec;152(Pt 12):3777–3786. doi: 10.1099/mic.0.29162-0

Fig. 3.

Fig. 3

Detection of L. interrogans surface labelling by LipL41 and LipL46 antisera and whole-cell ELISA. Wells of microtitre plates were coated with either L. interrogans sonicates (grey bars) or whole-cells (black bars). Antisera were diluted to give ELISA reactivity to L. interrogans sonicates in the 0·2–0·25 absorbance range at 450 nm. Antisera dilutions were identical in the sonicate and whole-cell ELISA experiments. Sonicate and whole-cell ELISA reactivities of antisera to LipL46 and the surface-exposed LipL41 were comparable, while sonicate ELISA reactivity exceeded whole-cell ELISA reactivity using antisera to the cytoplasmic heat-shock protein GroEL and the cytoplasmic membrane protein LipL31. The A450 values were obtained after subtracting the value for the control without antibody.