Reactivity of infected hamster sera with native and recombinant leptospiral proteins. (a, b) Immunoblots of total L. interrogans proteins (lane 1, 5×107 cells per lane) and recombinant LipL32, LipL41 and LipL46 (lanes 2–4, respectively, 200 μg per lane) separated by SDS-PAGE and probed with sera from hamsters infected with L. interrogans. His6-tagged recombinant proteins migrate slightly slower than native proteins. Serum from the first hamster (a) contained antibodies to all three proteins, while serum from the second hamster (b) contained antibodies to LipL46 only.