Figure 1. Treatment with SERMs inhibits proliferation of myelin-specific splenocytes.

Splenocytes from naïve female and male Vβ8.2/Vα4 TCR transgenic mice were stimulated with 2 μg/ml of MBP Ac1-11 peptide and treated with the indicated amounts of tamoxifen, raloxifene, 17β-estradiol, or 17α-estradiol for 72hrs. The data are presented as the percent change in proliferation (as measured by [³H]-thymidine incorporation) compared to vehicle treated control cultures (see Supplemental Fig. 1). The means and standard deviations of three separate experiments are shown.