Skip to main content
. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Clin Cancer Res. 2009 Mar 10;15(6):1954–1963. doi: 10.1158/1078-0432.CCR-08-1290

Figure 2. SPANX-B is expressed in human primary tumors.

Figure 2

(A) Immunohistochemistry staining of slides from paraffin embedded human primary melanoma (top panel) and non-small cell lung carcinoma (NSCLC, bottom panel); and T-TMA slides with human tumors (B) and normal tissues (C). The ANEA-I0117 Ab (SPANX-B) and control isotype-matched Ab (control IgG) were used at 1:500 dilution. (D) SPANX-B is mostly expressed in metastatic melanomas. Normalized signal intensity data for 77 genes previously identified as being associated with increased metastatic potential was averaged in each of 45 melanoma sample data sets (Mannheim data set) (12). This averaged profile (grey shaded line) was plotted against that of SPANX-B1 (black solid) across all samples and a positive correlation coefficient of 0.503 was calculated. Melanoma lines are labeled according to their cohort membership (12). Dotted lines mark the 95% confidence interval for the averaged profile. P-value is for comparisons between Cohorts A and C.