Table 5.

Relative expression levels of the p.N314 and p.D314 GALT messages in DN carriers

Sample	GALT genotype	Relative expression of p.N314 GALT message	Relative expression of p.D314 GALT message
1	p.D314 + (GTCA)2/N	0.525 ± 0.048	0.475 ± 0.048
2	p.D314 + (GTCA)2/N	0.573 ± 0.072	0.427 ± 0.072
3	p.D314 + (GTCA)2/N	0.541 ± 0.052	0.459 ± 0.052
4	p.D314 + (GTCA)2/N	0.530 ± 0.072	0.470 ± 0.072
Total		0.540 ± 0.011 (n = 4)	0.460 ± 0.011 (n = 4, P < 0.002)

These values were corrected for background cross-reactivity of the primers, which was measured at 1.5% for p.D314 signal and 3.5% for p.N314 signal.