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SP-IR in in vitro translation reactions. Poly(A) RNA from IL-1α-stimulated BM stroma was used as substrate in in vitro translation. Reactions contained cytosolic extract from malignant or normal mammary epithelial cells. Lanes: 1, no RNA; 2, Luciferase RNA; 3, poly(A) RNA with no extract; 4, T-47D extract; 5, BT-474 extract; 6, ZR-75–30 extract; 7, DU4475 extract; 8, MCF-12A extract. SP was immunoprecipitated and then characterized in Western blot.
