Table 3.
SP-IR by in vitro translation
| Cytosolic extracts | SP-IR | IL-1α | IL-6 | SCF |
|---|---|---|---|---|
| pg/μg poly(A)RNA
| ||||
| MDA-MB330 | 3,146 ± 45 | 20 ± 6 | 230 ± 22 | 6 ± 1 |
| T-47D | 2,750 ± 28 | 10 ± 2 | 435 ± 25 | 5 ± 2 |
| ZR-75-30 | 3,590 ± 42 | 5 ± 1 | 342 ± 34 | 8 ± 2 |
| BT-474 | 3,650 ± 36 | 12 ± 3 | 156 ± 21 | 2 ± 1 |
| DU4475 | 2,990 ± 30 | 8 ± 3 | 332 ± 18 | 10 ± 3 |
| Normal | ||||
| MCF-12A | 75 ± 20 | 15 ± 5 | 12 ± 4 | 5 ± 2 |
| Hs578Bst | 65 ± 5 | 18 ± 3 | 26 ± 6 | 4 ± 2 |
In vitro translation was performed in the presence or absence of cytosolic extracts from mammary epithelial cells. ELISA determined translated SP-IR/μg poly(A). Each point is the mean (±SD) of six experiments for SP-IR and three for IL-1α, IL-6, and SCF, poly(A)RNA from a different donor, and cell extracts from a different cell passage.