Table 4.
NK receptor antagonists on BC cell proliferation
| Cell lines | No. of colonies/103 cells (±SD)
|
|||
|---|---|---|---|---|
| Medium | CP-96,345 | SR 48968 | Both antagonists | |
| T-47D | 150 ± 5 | 60 ± 4 (40%) | 65 ± 7 (43%) | 30 ± 3 (20%) |
| BT-474 | 180 ± 10 | 70 ± 5 (39%) | 65 ± 8 (36%) | 34 ± 6 (19%) |
| ZR-75-30 | 165 ± 5 | 68 ± 8 (41%) | 60 ± 10 (36%) | 35 ± 5 (21%) |
| MDA-MB330 | 170 ± 8 | 65 ± 5 (38%) | 64 ± 4 (38%) | 38 ± 8 (22%) |
| DU4475 | 160 ± 8 | 63 ± 6 (39%) | 60 ± 5 (38%) | 30 ± 6 (19%) |
| Normal | ||||
| MCF-12A | 210 ± 12 | 198 ± 18 (94%) | 200 ± 9 (95%) | 220 ± 13 (104%) |
| Hs578Bst | 200 ± 11 | 201 ± 12 (100%) | 210 ± 11 | 205 ± 15 (103%) |
Clonogenic assays were performed in the presence or absence of 1 nM CP-96,345, SR 48968, or both. Colonies with more than 10 cells were enumerated and are the mean of four experiments (±SD). Each experimental point is the average of duplicate cultures. The percentages of colonies in medium alone are shown in parentheses.