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Reverse transcriptase (RT)-PCR assays showing expression of nanH and the housekeeping gene atpA in the type strains of Actinomyces oris (CCUG 34288; EU805602), and Actinomyces johnsonii (CCUG 34287; EU805600), Actinomyces naeslundii (CCUG 2238; EU805601) and Actinomyces viscosus (NCTC 10951; EU805603). Negative controls, omitting the RT in the reaction mix, yielded no amplicons from any strain.
