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. 2008 Dec 1;587(Pt 3):531–540. doi: 10.1113/jphysiol.2008.166678

Figure 1. diC8-PIP2 activates TRPC1 SOCs in rabbit portal vein myocytes.

Figure 1

A, bath application of 10 μm CPA evoked SOC activity in a cell-attached patch held at −80 mV. After excision into the inside-out configuration, SOC activity was potentiated by bath application of 10 μm diC8-PIP2. B, fixed level channel current amplitude histograms of SOC activity shown in A. Histograms could be fitted by the sum of several Gaussian curves showing the presence of more than one channel in the patch. Note that the peaks of the Gaussian curves have similar values in the absence (a) and presence of diC8-PIP2 (b) indicating that this phospholipid increased the activity of the same channel. C, bath application of 10 μm diC8-PIP2 activated channel currents in a quiescent inside-out patch held at −80 mV which were inhibited by anti-TRPC1 antibodies (Santa Cruz, 1: 200 dilution). D, diC8-PIP2-induced channel currents had a slope conductance of 1.9 pS between −120 and −50 mV.