Fig. 4.

Analysis of the relative levels of selected iTRAQ-differing proteins in tissues from adult DS model mice and human fetal DS tissue. A, Western blot showing total protein lysate from liver, kidney, testis, and heart from transchromosomic Tc1 (1) and normal littermate controls (2). The blot is stained with CSE1L, SERPINH1, ANXA5, TAGLN, and β-actin/GAPDH antibodies. The boxed area in the CSE1L blot is shown to the right in different contrast. B, Western blot showing total protein lysate from human fetal kidneys from DS (four different fetuses) (lanes 1) and from fetal age-matched euploid control (three different fetuses) (lanes 2). The blot is stained with SERPINH1 and β-actin.