Fig. 3.

Effect of synaptic activity and kinase inhibitors on phosphoproteins detected by MAPK phosphomotif 5557 antibody in cultured neurons. Hippocampal neurons (DIV21–25) were treated with various drugs as indicated. Total lysates were immunoblotted with MAPK phosphomotif antibody 5557 and with PSD-95 antibody (below). A, chronic treatment (16 h) with kinase inhibitors as indicated (50 μm PD98059, 5 μm SB203580, 20 μm SP600125, 10 μm roscovitine, and 20 μm SB4152286). B–D, time course with JNK inhibitor SP600125 (B), 100 μm glutamate, 50 μm NMDA, or 100 μm AMPA + 50 μm aminophosphonovaleric acid (APV) (C), and 50 μm bicuculline, 3 μm TTX, or 0.1 μm OA. D, major bands that change with several different treatments are marked with arrowheads or brackets and labeled with the approximate molecular mass (in kDa).