Table I.
Most promising potential MAPK substrates identified from cortical culture
5557-immunoprecipitated proteins identified from cortical culture were selected based on a high degree of induction upon okadaic acid treatment (log2(OA/untreated) > 2), presence of predicted MAPK phosphorylation site(s) (Scansite, medium stringency), and identification of at least two peptides by MS. For enrichment scores, an S.E. of 0 indicates that the log2 score is derived from a single quantified peptide only. The right column denotes proteins identified previously in the PSD (61). GeneID lists the NCBI gene number for the rat protein, whereas the NCBI gene name is taken from the human homolog. The full list of 410 identified proteins is provided in supplemental Table S3. LIM, Lin-11, Is1-1, Mec-3 zinc-binding domain; FERM = 4.1, Ezrin, Radixin, Moesin protein domain; PTPRF, protein tyrosine phosphatase, receptor type, f polypeptide.
| Accession no. | Human homolog | Description | Peptides | log2(OA/untreated) | Identified in PSD? |
|---|---|---|---|---|---|
| NP_077373.1 | ABI1 | Abl interactor 1 | 3 | 2.01 ± 0.26 | + |
| XP_217645.3 | ABLIM1 | Actin-binding LIM protein 1 long isoform | 9 | 3.34 ± 0.53 | + |
| NP_113968.1 | APBA2 | Amyloid β (A4) precursor protein-binding, family A, member 2 | 2 | 2.11 ± 0.50 | − |
| NP_446192.1 | ARHGEF7 | Rho guanine nucleotide exchange factor 7 | 6 | 2.11 ± 0.43 | + |
| XP_579639.1 | CEP170 | KARP-1 binding protein 1 (long form) | 21 | 2.74 ± 0.25 | − |
| NP_599243.1 | CEP170 | KARP-1 binding protein 1 (short form) | 14 | 3.06 ± 0.32 | − |
| NP_067718.1 | CNKSR2a | Connector enhancer of kinase suppressor of Ras 2; membrane-associated guanylate kinase-interacting protein 2 (MAGUIN2) | 5 | 2.61 ± 0.15 | + |
| XP_227658.3 | FNBP1L | Formin-binding protein 1-like, TOCA1 | 4 | 2.17 ± 0.70 | − |
| XP_240734.3 | FRMD4A | FERM domain-containing protein 4 | 25 | 3.49 ± 0.20 | − |
| XP_232212.3 | FRMD4B | FERM domain-containing 4B/GRP1 binding protein GRSP1 | 2 | 3.80 ± 0.03 | − |
| NP_445869.1 | GAB2 | Growth factor receptor-bound protein 2-associated protein 2 | 2 | 2.73 ± 01p | − |
| NP_067690.1 | HCN4 | Hyperpolarization-activated, cyclic nucleotide-gated K+ channel 4 | 3 | 2.87 ± 01p | − |
| XP_576105.1 | KIAA0284 | FAM68C, homolog of KARP-1; forkhead association domain | 11 | 2.19 ± 0.18 | + |
| XP_576303.1 | KIAA1688 | RhoGAP, MyTH4 and PRP4 domains | 7 | 3.24 ± 0.33 | + |
| XP_343352.2 | KIAA1731 | KIAA1731, no domains, no close homolog | 2 | 4.04 ± 1.01 | − |
| XP_220446.3 | LARP | La ribonucleoprotein domain family, member 1 | 6 | 2.86 ± 0.26 | − |
| NP_851603.1 | MAST1 | Microtubule-associated serine/threonine kinase 1 | 8 | 2.76 ± 0.16 | − |
| XP_343403.2 | NEO1 | Neogenin | 28 | 2.49 ± 0.19 | + |
| XP_215733.3 | PKP4a | Plakophilin 4 | 13 | 2.74 ± 0.26 | + |
| XP_341857.2 | PPFIA3 | PTPRF-interacting protein α3, liprin-α3 | 8 | 2.51 ± 0.41 | + |
| NP_113916.1 | SNAP91 | Synaptosomal-associated protein, 91 kDa | 17 | 2.83 ± 0.22 | + |
| NP_062251.1 | SNIPa | SNAP-25-interacting protein | 44 | 3.88 ± 0.21 | + |
| XP_233820.3 | SOS1 | Son of sevenless 1 | 21 | 3.57 ± 0.20 | + |
| NP_062021.1 | STRN | Striatin, calmodulin-binding protein | 2 | 3.10 ± 0.65 | + |
| NP_446199.2 | UBQLN1 | Ubiquilin 1 | 3 | 2.46 ± 0.80 | − |
| XP_228806.3 | UBQLN2 | Ubiquilin 2 | 11 | 2.71 ± 0.19 | − |
| XP_227405.2 | UBQLN4 | Ubiquilin 4, ataxin-1 ubiquitin-like interacting protein (A1Up) | 3 | 2.46 ± 0.80 | − |
| XP_227587.3 | WDR47 | KIAA0893; WD-40 domain | 8 | 2.15 ± 0.36 | + |
Protein is also listed in Table II.