Table II.
Most promising potential MAPK substrates identified from rat brain PSD
Shown is the list of 5557-immunoprecipitated proteins from rat brain PSD with at least three peptides identified by MS and predicted MAPK phosphorylation site(s) (Scansite, medium stringency). Columns are as in Table I. All 115 identified proteins are listed in supplemental Table S4. SH3, Src homology 3.
| Accession no. | Human homolog | Description | Peptides | Identified in PSD? |
|---|---|---|---|---|
| XP_575594.1 | AAK1 | AP2-associated kinase 1 | 6 | + |
| XP_215761.3 | ACTG1a | Actin, cytoplasmic 2, γ-actin | 13 | − |
| NP_058686.1 | ADD1a | Adducin 1 (α) | 29 | + |
| NP_036623.1 | ADD2a | Adducin 2 (β) | 4 | + |
| NP_062234.1 | ARC | Activity-regulated cytoskeletal associated protein | 3 | + |
| NP_068507.1 | CAMK2Ba | Calcium/calmodulin-dependent protein kinase II β subunit | 12 | + |
| NP_036651.1 | CAMK2Da | Calcium/calmodulin-dependent protein kinase II, δ | 4 | + |
| NP_598289.1 | CAMK2Ga | Calcium/calmodulin-dependent protein kinase II γ | 6 | + |
| NP_067718.1 | CNKSR2a | Connector enhancer of kinase suppressor of Ras 2; MAGUIN2 | 10 | + |
| NP_037064.1 | CRMP1a | Collapsin response mediator protein 1 | 3 | + |
| XP_001064375.1 | CTNND2a | Catenin, δ2 | 8 | + |
| NP_071618.1 | DLG2 | Chapsyn-110; discs, large homolog 2 | 8 | + |
| NP_062567.1 | DLG4 | PSD-95, postsynaptic density protein 95 | 27 | + |
| NP_775168.1 | DLGAP4 | Discs, large homolog-associated protein 4 | 3 | + |
| XP_577204.1 | FLJ22184a | No known homologues, KAR9 domain | 11 | − |
| XP_344572.2 | GPRIN1a | G-protein-regulated inducer of neurite outgrowth 1 | 4 | − |
| NP_476480.1 | HNRPU | Heterogeneous nuclear ribonucleoprotein U | 3 | − |
| NP_113895.1 | HOMER1a | Homer 1 | 7 | − |
| NP_068635.1 | HSPA2a | Heat shock protein 2 | 5 | − |
| XP_224824.3 | HSPA8a | Heat shock protein 8 | 3 | − |
| NP_476483.1 | KIAA1365 | Densin-180 | 3 | − |
| NP_446073.1 | MAGI2a | Membrane-associated guanylate kinase, WW and PDZ domain-containing 2 | 12 | − |
| XP_215469.2 | MAP1Ba | microtubule-associated protein 1B | 13 | + |
| NP_037198.1 | MAP2a | Microtubule-associated protein 2 | 30 | + |
| NP_058900.1 | MAP6a | Microtubule-associated protein 6 | 4 | + |
| NP_058722.1 | MBP | Myelin basic protein | 6 | + |
| XP_574117.1 | MYO7Ba | Myosin-VIIb | 13 | + |
| NP_001009967.1 | PIP5K1Ca | Phosphatidylinositol-4-phosphate 5-kinase, type I, γ | 5 | + |
| XP_215733.3 | PKP4a | Plakophilin 4 | 14 | + |
| NP_445926.1 | PPP1R9Ba | Protein phosphatase 1, regulatory subunit 9B, spinophilin | 7 | + |
| XP_233240.3 | SGIP1a | SH3 domain GRB2-like (endophilin) interacting protein 1 | 12 | − |
| NP_067708.1 | SHANK3 | SH3/ankyrin domain gene 3 | 5 | + |
| NP_062251.1 | SNIPa | SNAP-25-interacting protein | 32 | + |
| NP_062006.1 | SYN1 | Synapsin 1 | 16 | + |
| NP_062032.1 | SYN2a | Synapsin 2 | 4 | + |
| NP_851606.1 | SYNGAP1 | Synaptic Ras GTPase-activating protein 1 | 13 | + |
| NP_067727.1 | SYNPO | Synaptopodin | 7 | + |
| NP_775125.1 | TUBBa | Tubulin, β5 | 13 | − |
| NP_954525.1 | TUBB2Ca | Tubulin, β2c | 14 | − |
| XP_579666.1 | TUBB3a | Tubulin, β3 | 10 | − |
a
Protein that was also identified from cortical culture (regardless of its enrichment score).