Fig. 1.

Single and mixed clone infections of the rodent malaria parasite P. y. yoelii 17XYM and 33XC in mice. (A) Parasitemias are shown during the course of single clone infections with either 17XYM (red solid line) or 33XC (blue solid line) measured on thin blood smears stained with Giemsa's stain. (B and C) Clone specific parasitemias (17XYM, red dashed line; 33XC, blue dashed line) and absolute parasitemias (black solid line) are shown in mixed clone infections with parasites of 17XYM and 33XC in proportions of 1:1 and 1:9 (17XYM:33XC), respectively. The clone specific parasitemias in the mixed infections were calculated from the absolute parasitemias measured on thin blood smears stained with Giemsa's stain and from proportions of 17XYM and 33XC in the mixtures as measured by using Pyrosequencing. Error bars give the 95% confidence intervals of the means. Asterisks (*) represent statistically significant differences (P ≪ 0.01) in parasitemia between 17XYM and 33XC.