TABLE I.

Comparison of the peptides detected by ESI-MS from uroplakins II and III after conventional in-gel digestion or on-membrane digestion after Western blot analysis

m/z	Sequence	Scorea
		IGb	OM/NC-freec
Uroplakin II
1033	AESIGLAMAR + Ox(M)d	31	13
1092	EIPMSTFPR + Ox(M)	30	23
1145	KAESIGLAMAR + Ox(M)	34	75
1148	YYSYLVTK	39	31
1462	LSAYQVTNLAPGTK	92	91
1651	ELVSVVDSGSGFTVTR	79	96
Uroplakin III
1261	ASEILNAYLIR	60	81
1491	LTLYSAIDTWPGR	N.D.e	81
1496	SLGTSEPSYTSVNR	78	32
1506	TPLSSTFQQTQGGR	89	104
2025	GDADGATSHDSQITQEAVPK	N.D.	96

^aMascot score.

^bConventional in-gel digestion.

^cOn-membrane digestion carried out after Western blot analysis.

^dOxidation of Methionine.

^eND, not detected.