Table 1.
Production of TSST-1 evaluated by Western blotting.
| No. | Strain | μg/ml | No. | Strain | μg/ml |
|---|---|---|---|---|---|
| 1 | N315 | 3.5 ± 0.22 | 18 | 2680 | 1.4 ± 0.19 |
| 2 | A36 | 14 ± 1.01 | 19 | 2681 | 1.3 ± 0.05 |
| 3 | 3429 | 5 ± 0.12 | 20 | 2682 | 1.0 ± 0.25 |
| 4 | 3472 | 1.3 ± 0.31 | 21 | 2683 | 1.0 ± 0.01 |
| 5 | 3337 | 1.1 ± 0.20 | 22 | 2684 | 0.8 ± 0.02 |
| 6 | 1785 | 1.2 ± 0.02 | 23 | 2685 | 1.6 ± 0.23 |
| 7 | 2271 | 2.0 ± 0.03 | 24 | 2686 | 2.0 ± 0.18 |
| 8 | 3281 | 4.0 ± 0.22 | 25 | 2687 | 1.6 ± 0.22 |
| 9 | 2932 | 2.8 ± 0.19 | 26 | 2688 | 7.6 ± 0.07 |
| 10 | 3543 | 14 ± 1.21 | 27 | 2689 | 9.8 ± 0.28 |
| 11 | 3573 | 12 ± 0.20 | 28 | 2690 | 3.1 ± 0.16 |
| 12 | V432 | 7.0 ± 0.25 | 29 | 2701 | 5.0 ± 1.12 |
| 13 | V637 | 7.6 ± 0.30 | 30 | 2702 | 2.8 ± 0.23 |
| 14 | V666 | 5.2 ± 0.11 | 31 | 2165 | 4.0 ± 0.13 |
| 15 | V700 | 8.0 ± 0.21 | 32 | 3624 | 1.0 ± 0.19 |
| 16 | V723 | 1.3 ± 0.34 | 33 | 3878 | 2.2 ± 0.20 |
| 17 | V694 | 4.0 ± 0.22 | 34 | 3890 | 6.4 ± 0.08 |
Strain 1, genome strain. Strains 2 to 34 were randomly selected clinical MRSA isolates that were all tst-positive and assigned to agr class 2 by PCR. Amounts of TSST-1 varied among strains and ranged from 0.8 to 14 μg/ml.