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Characterization of Nef-inducible Jurkat T lymphoid cell lines. (A) Nef-specific Western blot analysis of indicated cells grown in the presence or absence of tetracycline for 5 days. Nef levels in nef-induced (+), control (−), and HIV-1-infected (HIV) Jurkat cells are compared underneath. (B) Cell surface levels of CD4 and MHC-I measured by fluorescence-activated cell sorter analysis. Result is representative of the two clones tested in each case.
