Figure 7.

Nef increases NFAT and HIV-1 LTR transcriptional responsiveness in transiently transfected Jurkat T cells. D4 Jurkat T cells were cotransfected with NFAT (A) or HIV-1 LTR (B) luciferase plasmids and either an empty vector (pTet-Splice) or the indicated Nef expression plasmids, plus pRL-TK as an internal control. Twenty-four hours later, cells either were left unstimulated or were treated with 3 μg/ml anti-CD3 or CD28 antibodies for fourteen hours and were analyzed for luciferase activity. Ratios of values obtained with vs. without stimulation are shown, averaging two independent transfections.