Table 1.

2D-DIGE experimental design

Gel number	Cy2	Cy3	Cy5
1	Pooled standard	Sample 1	Sample 2
2	Pooled standard	Sample 3	Sample 4
3	Pooled standard	Sample 5	-

For DIGE minimal labelling, 25 μg of protein sample were mixed with 100 pmol CyDye (GE Healthcare). Every gel included 2 different samples, labelled with a different CyDye, and a Cy2-labelled internal standard, which is a pool of all the samples within the experiment.