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. 2009 Mar 30;8:18. doi: 10.1186/1475-2859-8-18

Figure 6.

Figure 6

Semi-quantitative analysis of the SACE 1456-SACE 1459-specific transcripts by RT real time PCR. (A) Genetic map of the SACE 1456-SACE 1459 genetic cluster coding for enzymes involved in the valine catabolic pathway (B). (B) Possible metabolic pathways leading to propionyl-CoA and 2-methylmalonil-CoA, the two building blocks for biosynthesis of the erythromycin precursor 6-deoxyerythronolide B. (C) Semi-quantitative analysis of SACE 1456-SACE 1459-specific transcripts by RT real-time PCR. The RNAs were extracted from S. erythraea NRRL2338 and rif derivatives rif1 and rif6 grown in R3/1 medium up to phase a. Results were normalized to 16S rRNA levels. Transcript levels of S. erythraea NRRL2338 were arbitrarily given a value of 1. Data are shown as mean ± standard deviation from three independent experiments, each with triplicate samples, using distinct cDNA preparations for each RNA sample. The Student's t-test was used for statistical analysis. Statistically significant differences between values from S. erythraea NRRL2338 and rif mutants (asterisks) are declared at a p value < 0.05.