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. 2009 Mar 26;6:12. doi: 10.1186/1742-2094-6-12

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Copyright © 2009 Akar and Feinstein; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of inflammatory activation on SRGs, NOS2, and C/EBPβ mRNA levels in astrocytes. Primary rat astrocytes were treated with LPS (L) or LPS+NA (LN) for 4 hours. Total mRNA was converted to cDNA, and amplified using primers for (A) SUMO-1, Ubc9, and SENP1; and (B) NOS2 and C/EBPβ. PCR products were separated through 2% agarose gels, and the band intensities were quantified using ImageJ software. The data is the mRNA level measured in treated samples compared to non-treated control samples, and normalized to values for β-actin measured in the same samples. Data is the mean ± sd of n = 3 samples in each group. **, p < 0.01; ***, p < 0.001 L vs control or LN vs L (unpaired t-test).