Table 1.
Binding capacity of Melan-A/MART-1 peptides for HLA-DRB1*0401 and DRB1*0404
| Melan-A/MART-1 peptide: amino acid positions | Sequence | Algorithm score, 0–10 | Binding capacity, IC50, nM
|
|
|---|---|---|---|---|
| DRB1*0401 | DRB1*0404 | |||
| 16–37 | GHGHSYTTAEEAAGIGILTVIL | 3 | >30,000 | 27,000 |
| 27–40 | AAGIGILTVILGVL | 2 | >30,000 | 83 |
| 29–50 | GIGILTVILGVLLLIGCWYCRR | 5 | >30,000 | 13,991 |
| 43–57 | IGCWYCRRRNGYRAL | 3 | 11,912 | 17,726 |
| 51–73 | RNGYRALMDKSLHVGTQCALTRR | 3 | 3,268 | 4,682 |
| 102–116 | PAYEKLSAEQSPPPY | 5 | 3,043 | >30,000 |
Peptide sequences are provided using single-letter amino acid designations. Peptide-binding algorithm (11, 12) scores for the highest-scoring embedded 9-mer sequences (underlined) are indicated for each peptide evaluated. Binding capacity was estimated in a competitive fashion against the non-natural YARFQSQTTLKQKT reference peptide as indicated in Materials and Methods and ref. 14.