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. 2009 Mar 19;9:22. doi: 10.1186/1472-6750-9-22

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Copyright © 2009 Bortesi et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Assessment of IL-10 integrity and dimerization. Viral (a) and murine (b) IL-10, purified from transgenic tobacco leaf extracts by IMAC, were analyzed by immunoblotting after separation by non-reducing (left panels) and reducing (right panels) SDS-PAGE. Commercial recombinant viral IL-10 produced in E. coli (rvIL-10) or murine IL-10 produced in insect cells (rmIL-10) were used as controls, and different amounts of plant-derived IL-10 were loaded, as indicated on top of each lane. (c) Elution profile of plant-derived mIL-10 from a Superdex 200 column showing a single peak which, compared to the elution volumes of standard reference molecules on the same columns (not shown), matches with the expected size of the dimer. Detection of IL-10 in the elution fractions was carried out by ELISA.