Table 1.

Comparison of nucleosome positions on SNR6, mapped in this study with those reported in other studies

No.	Position (bps) This study	Similar to position (bp)	Comments	Ref. No.
1.	-545 to -368	-537 to -367	In vivo, in the presence of bound TFIIIC	39
2.	-368 to -78	-367 to -214	In vivo, in the presence of bound TFIIIC	39
3.	-656 to -453		In vitro, sequence-directed
4.	-453 to -241		In vitro, sequence-directed
5.	-241 to -78	-240 to -70	In vivo, in the presence of bound TFIIIC	37
6.	-125 to +25	-194 to -16	In vivo, in the absence of bound TFIIIC	39
		-120 to +28	In vivo, under repression	37
7.	-110 to +45	-116 to +40	Predicted	26
8.	-90 to +65	Positions 8–10 together as bp -78 to + 110 in the plasmid
9.	-60 to +95	p-539H6, Figure 1B, sequence-directed.
10.	-40 to +115	Positions 9–15 together as bp -50 to + 316 in the plasmid
11.	-20 to +125	pCS6, Figure 1C, sequence-directed.
12.	+51 to +196	+48 to +192	In vitro, TFIIIC-dependent	3
		+94 to +198	In vivo, in the presence of bound TFIIIC	59
13.	+71 to +216	+71 to +227	Predicted	26
		+85 to +206	In vivo, in the absence of bound TFIIIC	39
14.	+91 to +236		In vitro, sequence-directed
15.	+101 to +256		In vitro, sequence-directed

All 15 positions in Column 2 are sequence-directed as nucleosomes are assembled in the absence of any transcription or chromatin assembly factors. Positions 1 to 5 are mapped by the low-resolution technique (Figure 1B) and fall in the upstream region; the rest of the positions on the gene region (6 to 15) are mapped by the higher resolution Exo III analysis (Figures 5 and 6) in this study. Positions 3 to 5, 8, 9, 14 and 15 represent the register R1 while R2 is constituted by the positions 1, 2 (or 3, 4), 6, 12 and 13. Arrangement of nucleosomes in active state R3 is generated by the positions 1, 2 (or 3, 4), 5 and 12 giving a nucleosome-free region flanked by positioned nucleosomes.