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Constitutive (pS7PPX1) and regulated (pBADPPX1) overexpression of yeast PPX1 in Pseudomonas sp. B4 cells. (A, B) Westtern-blotting using anti His6t mAb and (C, D) thin layer chromatography (TLC) of exopolyphosphatase activity products. (E) Phosphate (Pi) standard curve to determine exopolyphosphatase activity. Cells were from exponential (Ex) or stationary (St) phases.
