Table 1.
Strains | Characteristics | Reference |
Pseudomonas sp. B4 constitutive polyP(-) | Pseudomonas sp. B4 transfomant with pS7PPX1 plasmid | This study |
Pseudomonas sp. B4 constitutive control |
Pseudomonas sp. B4 transfomant with pMLS7 plasmid |
This study |
Pseudomonas sp. B4 regulated polyP(-) | Pseudomonas sp. B4 transfomant with pBADPPX1 plasmid | This study |
Pseudomonas sp. B4 regulated control | Pseudomonas sp. B4 transfomant with pMLBAD plasmid | This study |
Escherichia coli NR100 | M15 (pREP) derivative with pQE30PPK plasmid | [24] |
Plasmids | ||
pGEM®-T-easy | T-vector cloning system | Promega |
pMLBAD | Broad-host range regulated expression vector | [22] |
pMLS7 | Broad-host range constitutive expression vector | [22] |
pTYPPX1 | pGEM®-T-easy with PPX1 gene from S. cerevisiae. (His6 tag) | This study |
pS7PPX1 | pMLS7 with PPX1 gene from S. cerevisiae. (His6 tag) | This study |
pBADPPX1 | pMLBAD with PPX1 gene from S. cerevisiae. (His6 tag) | This study |