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. 2009 Mar 26;2:50. doi: 10.1186/1756-0500-2-50

Table 1.

Strains and plasmids used in this study

Strains Characteristics Reference
Pseudomonas sp. B4 constitutive polyP(-) Pseudomonas sp. B4 transfomant with pS7PPX1 plasmid This study
Pseudomonas sp. B4 constitutive control Pseudomonas sp. B4 transfomant with
pMLS7 plasmid
This study
Pseudomonas sp. B4 regulated polyP(-) Pseudomonas sp. B4 transfomant with pBADPPX1 plasmid This study
Pseudomonas sp. B4 regulated control Pseudomonas sp. B4 transfomant with pMLBAD plasmid This study
Escherichia coli NR100 M15 (pREP) derivative with pQE30PPK plasmid [24]
Plasmids
pGEM®-T-easy T-vector cloning system Promega
pMLBAD Broad-host range regulated expression vector [22]
pMLS7 Broad-host range constitutive expression vector [22]
pTYPPX1 pGEM®-T-easy with PPX1 gene from S. cerevisiae. (His6 tag) This study
pS7PPX1 pMLS7 with PPX1 gene from S. cerevisiae. (His6 tag) This study
pBADPPX1 pMLBAD with PPX1 gene from S. cerevisiae. (His6 tag) This study