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Loss of Urm1p and Uba4p is synthetically lethal with cla4Δ. Strains SY3362 (cla4Δ), SY3805 (uba4Δ), SY3806 (urm1Δ), SY3807 (uba4Δ cla4Δ), SY3808 (urm1Δ cla4Δ), and SY3754 (ste20Δ cla4Δ) harboring pRS316ADE8CLA4 were streaked on YEPD plates and incubated at 30°C for 2 d. Plates were then replica-plated to 5-FOA and incubated at 30°C for 3 d.
