Fig. 1.

Immunoblotting (11% SDS-PAGE). Affinity-purified primary antibody against GLT1 (lanes 1–3), EAAC1 (lanes 4–6), GLAST (lanes 7–9) and GCPII (lanes 10–12) were used. The amount of total protein samples loaded per lane was 40 µg. All three glutamate transporters and GCPII were expressed in both the sciatic nerve and DRG, at the same expected molecular weight as the positive control represented by brain homogenate (GLT1 = 68 kDa, EAAC1 = 70 kDa, GLAST = 60 kDa and GCPII = 85 kDa). Actin (42 kDa) was used for the normalized quantitative densitometric analysis of the bands.