Table 2.
Antigens used for adsorption controls. The antisera were adsorbed at highest working dilution employed for light microscopy. Homologous antigen (antigens which induced antiserum formation) was added to antiserum up to 25 µg mL−1, heterologous antigen (antigen which can react with an antiserum but is not the one that induced its formation) was added to antiserum up to 50 µg mL−1
| Antisera adsorbed | Homologous antigen | Heterologous antigen |
|---|---|---|
| GDNF (D-20) | GDNF (sc-328 P Santa Cruz Biotechnology) | Neurturin (sc-6362 P Santa Cruz Biotechnology), persephin (sc-8684 P Santa Cruz Biotechnology), artemin (sc-9330 P Santa Cruz Biotechnology) |
| GFR alpha 1 (H-70) | GFR alpha1 (sc-6157 P Santa Cruz Biotechnology) | GFRalpha 2 (sc-7414 P Santa Cruz Biotechnology); GFRalpha 3 (sc-9340 P Santa Cruz Biotechnology); GFRalpha 4 (sc-46987 Santa Cruz Biotechnology) |
| RET (C-19) | RET (sc-167 P Santa Cruz Biotechnology) | Fibroblast growth factor receptor 1 (sc-121 P Santa Cruz Biotechnology) |