Figure 2.

Representative hyphal morphology mutants. The wild-type parent (A) and various mutants (B-BB) were grown at 25°C on minimal agar medium without added osmotic stabilizer and then shifted to restrictive temperature (39°C) for 10 h. Pictures were taken of hyphae growing embedded in agar with the exception of panel X, which shows hyphae growing on an agar surface. (The pod-9 mutant phenotype was similar, but more difficult to photograph, when the hyphae were growing embedded in agar.) All mutants shown here displayed wild-type or nearly wild-type growth at 25°C. Other mutants had phenotypes more-or-less similar to those shown here (see Table 1). Bars, 20 μm.