Figure 10.

Effect of nocodazole treatment on cell surface expression of CFTR and chloride efflux in T84 cell monolayers. Enzyme linked CFTR surface expression assays were performed on forskolin stimulated T84 cell monolayers with and without treatment with 33 μM nocodazole for 3 h. CFTR expression is represented by absorbance at 655 nm, normalized to the mean absorbance of the control group within individual experiments. Six monolayers were examined in each group. The mean ± SD for each group is depicted. (Control 1.00 ± 0.29, Nocodazole 0.92 ± 0.25; p = 0.64). (B) MQAE chloride efflux assays were performed on control T84 cell monolayers and T84 cell monolayers treated with nocodazole 33 μM for 3 h. Cumulative chloride efflux at 15 min is represented by the mean percentage increase in fluorescence from baseline, normalized to the mean percentage increase of the control group, within individual experiments. Ten monolayers were examined in each group. The mean ± SD for each group is depicted. (Control 1.00 ± 0.22, Nocodazole 1.01 ± 0.43; p = 0.93)