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. 2003 Nov;14(11):4365–4375. doi: 10.1091/mbc.E03-03-0169

Figure 2.

Figure 2.

Tropomyosin antibody specificity. Tropomyosin antibody specificity in T84 cells and human fibroblasts are shown in Western blots. The specificities of 311 in T84 cells (left) and fibroblasts (right) are shown in A and the specificities of αf9d and CG3 antibodies are shown in B and C respectively. The 311 antibody detects Tm6 (40 kDa), Tm2 (36 kDa) and Tm3 (34 kDa) in human fibroblasts. Tm2 is absent and Tm3 is quite minor in T84 cells. Tm1 (36 kDa) was absent from both T84 cells and human fibroblasts (A). αf9d detects Tm6 (40 kDa), Tm3 (34 kDa), Tm5a (30 kDa) and Tm5b (30 kDa) in T84 cells (B). CG3 antibody detects 11 possible isoforms (Tm5NM1–11) that comigrate at 30 kDa and shows a single band in T84 cells composed of an unknown number of isoforms (C).