Figure 6.

Localization of tropomyosin isoforms in T84 cells after treatment with jasplakinolide or nocodazole. Immunofluorescent confocal microscopy images of T84 cells stained for tropomyosin isoforms 10 min (A–D)or 7 d (E–H) after cell seeding. All images are in the vertical (xz) plane. Cells on the left (A, C, E, and G) are stained with 311 antibody (Tm 3,6) and cells on the right (B, D, F, and H) are stained with αf9d antibody (Tm 3, 5a, 5b, 6). (A and B) Cells treated with 1 μM jasplakinolide 10 min before plating. (C and D) Cells treated with 33 μM nocodazole 10 min before plating. (E and F) T84 cell monolayers treated with 20 μM cytochalasin D for 3 h. The arrows indicate a T84 cell in suspension with circumferential staining of both 311 and αf9d antibody. (G and H) T84 cell monolayers treated with DMSO alone. Bar, 10 μm.